Research has shown that childhood asthma is associated with a two to three times higher chance of developing an internalizing disorder such as anxiety or depression, but the exact reasons for this have remained a mystery.
In a new mouse study at Pennsylvania State University, the researchers suggest that persistent lung inflammation may be one possible explanation for an increased risk of anxiety. They discovered that exposure to allergens early in life was linked with persistent lung inflammation and also tied to changes in gene expression related to stress and serotonin function. They also found that females were more likely to have inflamed lungs for a longer period of time.
“The idea of studying this link between asthma and anxiety is a pretty new area, and right now we don’t know what the connection is,” said Dr. Sonia Cavigelli, associate professor of biobehavioral health. “What we saw in the mice was that attacks of labored breathing may cause short-term anxiety, but that long-term effects may be due to lasting lung inflammation.”
The researchers say that finding the root cause of this connection is difficult because in addition to the biological aspects of asthma, there are also several social and environmental factors that could contribute to anxiety in humans. For example, air pollution or a parent’s anxiety about their child’s asthma could also influence the child’s risk for anxiety.
“With the mice, we can look at the different components of asthma, like the lung inflammation or the airway constriction,” said Jasmine Caulfield, graduate student in neuroscience and lead author on the study.
“A person who’s having an asthma attack may have inflammation in their lungs and labored breathing at the same time, so you can’t separate which is contributing to later outcomes. But in mice, we can isolate these variables and try to see what is causing these anxiety symptoms.”
To help differentiate between these potential causes, the researchers studied a total of 98 mice, divided into four groups: one with airway inflammation due to dust mite exposure; one that experienced episodes of labored breathing; one that experienced both conditions; and one that experienced neither, as a control.
The findings reveal that three months after being exposed to the allergen, the mice still had lung inflammation and mucus, suggesting that even when allergy triggers are removed, there are lasting effects in the lungs long into adulthood.
“We originally thought that once the allergen was removed, the lungs would clear themselves of inflammation relatively quickly,” Cavigelli said. “If this translates to humans, it may suggest that if you grow up exposed to an allergen that you’re reacting to, even if you get over that, you might still have these subtle, long-term changes in lung inflammation.”
They also discovered that the mice that were exposed to the allergen and developed these changes in lung function also had changes in gene expression in brain areas that help regulate stress and serotonin.
“It makes sense to us because while labored breathing events may be scary and cause anxiety in the short term, it’s the inflammation in the airways that persists into adulthood,” Caulfield said. “So, it would make sense that long-term anxiety is linked with this long-term physical symptom.”
The researchers also found differences between male and female mice.
“In this study, the female mice had more inflammation in their lungs than the male mice three months after exposure to the allergen,” Caulfield said. “In humans, girls are more likely to have persistent asthma while boys are more likely to outgrow it, so our animal model seems to map onto what we see in humans.”
The new findings are published in the journal Frontiers in Behavioral Neuroscience.
Source: Penn State