Researchers studying fruit flies report identifying a key molecule that triggers the chemical processes of memory formation.
The finding, published in the journal Frontiers in Neural Circuits, is important as the molecule may represent a new target for therapeutic interventions to reverse memory loss.
Scientists at the University of Bristol sought to better understand the mechanisms that enable the formation of memories by studying the molecular changes in the hippocampus, a brain area critically involved in learning.
Researchers said previous studies have shown that our ability to learn and form memories is due to an increase in synaptic communication called long term potentiation, or LTP. This communication is initiated through a chemical process triggered by calcium entering brain cells and activating a key enzyme called ‘Ca2+ responsive kinase’ (CaMKII).
Once this protein is activated by calcium it triggers a switch in its own activity enabling it to remain active even after the calcium has gone. This special ability of CaMKII to maintain its own activity has been termed “the molecular memory switch.”
Until now, the question still remained as to what triggers this chemical process in our brain that allows us to learn and form long-term memories.
The research team conducted experiments using the common fruit fly (Drosophila) to analyze and identify the molecular mechanisms behind this switch. Using advanced molecular genetic techniques that allowed them to temporarily inhibit the flies’ memory the team were able to identify a gene called CASK as the synaptic molecule regulating this “memory switch.”
James Hodge, Ph.D., the study’s lead author, said: “Fruit flies are remarkably compatible for this type of study as they possess similar neuronal function and neural responses to humans. Although small they are very smart — for instance, they can land on the ceiling and detect that the fruit in your fruit bowl has gone off before you can.”
“In experiments whereby we tested the flies’ learning and memory ability, involving two odors presented to the flies with one associated with a mild shock, we found that around 90 per cent were able to learn the correct choice remembering to avoid the odor associated with the shock.
“Five lessons of the odor with punishment made the fly remember to avoid that odor for between 24 hours and a week, which is a long time for an insect that only lives a couple of months.”
By localizing the function of these key molecules researchers found that the flies lacking these genes showed disrupted memory formation. In repeat memory tests those lacking these key genes were shown to have no ability to remember at three hours (mid-term memory) and 24 hours (long-term memory) although their initial learning or short-term memory wasn’t affected.
Finally, the team introduced a copy of the human CASK gene — 80 per cent identical to the fly CASK gene — into the genome of a fly that completely lacked its own CASK gene and was therefore not usually able to remember. The researchers found that flies that had a copy of the human CASK gene could remember like a normal wild-type fly.
Hodge, from the university’s School of Physiology and Pharmacology, said: “Research into memory is particularly important as it gives us our sense of identity, and deficits in learning and memory occur in many diseases, injuries and during aging.”
“CASK’s control of CaMKII molecular memory switch is clearly a critical step in how memories are written into neurons in the brain. These findings not only pave the way for to developing new therapies which reverse the effects of memory loss but also prove the compatibility of Drosophila to model these diseases in the lab and screen for new drugs to treat these diseases.
“Furthermore, this work provides an important insight into how brains have evolved their huge capacity to acquire and store information.”
Source: University of Bristol