Antibodies May Slow Alzheimer’s
Emerging research has identified a set of antibodies that attack substances that aggregate to form plaques, such as those found in Alzheimer’s patients.
The antibodies have been identified in the blood and cerebrospinal fluid of healthy people.
Levels of these antibodies decline with age and, in Alzheimer’s patients, with increasing progression of the disease.
These findings by Stanford University School of Medicine researchers and outside collaborators, described in a paper to be published in the journal Proceedings of the National Academy of Sciences, raise the possibility that many of us are carrying antibodies in our blood that could be playing a role in staving off or slowing the progression of Alzheimer’s disease.
This seems to be true even when we are young and healthy and would presumably have had little or no exposure to the substances that build up in the brain to cause this disorder.
Alzheimer’s disease is characterized by the buildup of amyloid plaques in the brain. These are large aggregations of a protein breakdown product, or peptide, called A-beta. Many experiments have shown that immunization with A-beta can reduce the formation of amyloid plaques.
Clinical trials now under way are exploring whether this can safely produce cognitive benefits in Alzheimer’s patients, while other trials are treating patients directly with antibodies to A-beta.
But A-beta is a slippery character. A number of different modified, mutated, or metabolized forms of this peptide may also be involved in the disease. Furthermore, it is believed that more than the plaques themselves (which are also found in the brains of people with no Alzheimer’s symptoms), it is smaller aggregations of a few A-beta molecules, called oligomers, which are most toxic to neurons.
“Other studies have found antibodies against A-beta, but nobody has ever done a large-scale analysis using hundreds of different samples and almost a hundred different peptides to look for what’s already in people’s bodies,” said the paper’s first author, Markus Britschgi, PhD, an instructor working as a researcher in the laboratory of Tony Wyss-Coray, PhD.
Britschgi, Wyss-Coray, and their colleagues used a microarray technology in which a large number of different peptides are affixed to pixels on a microchip, which signals binding of those peptides by antibodies.
This technology was developed in a neighboring lab by another co-author of the paper, William Robinson, MD, assistant professor of rheumatology, and other Stanford scientists.
The investigators customized microarrays containing close to 100 different peptides apiece, including A-beta and several of its metabolized, modified, and mutant forms. The peptides were displayed in various degrees of aggregation, ranging from single molecules to small assemblages, or oligomers, to denser aggregates called fibrils.
Also included on the chip were certain peptides whose constituent amino-acid sequences are unrelated to A-beta’s but that are capable of aggregating to form other, rare plaque-associated dementias.
The researchers incubated these chips with blood samples from more than 250 individuals, who were between 21 and 89 years old, some with Alzheimer’s disease and others without it. They observed antibodies targeting many forms and aggregation-states of A-beta in both healthy and diseased subjects’ blood, with antibodies to oligomers showing the most immunoreactivity.
They then showed that overall levels of these antibodies decline with age and, in those with Alzheimer’s, with advancing stages of the disease.
“This was the first study to show an age-related decline in the levels of these antibodies,” said Britschgi.
Nauert PhD, R. (2015). Antibodies May Slow Alzheimer’s. Psych Central. Retrieved on March 20, 2018, from https://psychcentral.com/news/2009/07/10/antibodies-may-slow-alzheimers/7030.html