Anti-adhesive layers leave no hope for insects



The pitfall trap of the pitcher plant Nepenthes alata. Background: the image from a scanning electron microscope of the upper and lower wax layers. The diagrams show how the two wax layers reduce the adhesive ability of insects. The upper layer contaminates the insects' adhesive pads, whereas the lower layer decreases the amount of contact between the adhesive hairs of the foot and the substrate. Image: Max Planck Institute for Metals Research

Plants are able, using organic substances, to achieve effects that we otherwise mostly know only from technical materials. One example of this is the carnivorous pitcher plant, as researchers from the Max Planck Institute for Metals Research and the University of Hohenheim have shown. These plants catch insects and hold them using traps with a double layer of crystalline wax. The upper layer has crystalloids which contaminate the attachment organs that insects use to adhere themselves to surfaces. The lower layer additionally reduces the contact area between the insect feet and plant surface. The insects thus slip into the pitcher-shaped traps, where they are digested (The Journal of Experimental Biology, December 2005). These results provide ideas for further developments of technological anti-adhesive surfaces.

One highly specialised ecological group of plants is carnivores - meat-eaters. In order to obtain nutrients such as nitrogen and phosphorous, which may be lacking in the soil, carnivorous plants catch and digest small animals, primarily insects. These plants have evolved particular organs to catch their prey. Different types of carnivores bear traps with different morphologies and catch-mechanisms.

The pitfall trap of the tropical pitcher plant Nepenthes belongs to the group with passive traps. In this group, the plant does not move in order to catch animals. Although the origins of the pitchers lie in leaf organs, the structures that originate from the leaves are not leaf-like. Nepenthes pitchers are organised in a complex way, with a lid, a peristome (a ring around the pitcher's entrance), and slippery and digestive zones, the latter containing a supply of digestive fluid. These pitchers draw in insects, hold them, and finally digest them.

The slippery zone is very important to successful trappings. It is covered by a layer of crystalline wax on which insects lose their footing and slide down into the digestive fluid. Until now, studies have focused either on the wax structure or the behaviour of insects in the trap.

The Stuttgart researchers have investigated the micromorphology, chemical composition, and mechanical characteristics of the wax and combined them with experiments concerning insect behaviour. The wax cover is made of two layers that are different in structure, chemical composition, hardness, and elasticity. The wax layers reduce the adhesive ability of insects in two very different ways.

The top layer is made of single, irregular, 30-50 nanometre thick platelets standing more or less perpendicular to the surface of the pitcher's wall. They are of somewhat random orientation without a clear pattern. The platelets bear a small "stalk" connected to the lower layer of wax.

The lower layer is similar to foam. It is made of connected membrane-like platelets, which stick out from the surface at sharp angles and do not show any clear orientation. The hardness and elasticity of both layers are different by more than an order of magnitude; the upper wax layer is much softer and more elastic than the lower one.

Laboratory experiments with lady-bird beetles Adalia bipunctata showed that the wax layers - compared to glass or de-waxed pitcher walls - significantly reduce the adhesive ability of the insects. The upper layer contaminates the insects' feet and makes them less adhesive. The lower layer reduces the contact area between the feet and the trap.

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Original work:
Gorb E., Haas K., Henrich A., Enders S., Barbakadze N. and Gorb S. Composite structure of the crystalline epicuticular wax layer of the slippery zone in the pitchers of the carnivorous plant Nepenthes alata and its effect on insect attachment. The Journal of Experimental Biology, 2005, vol. 208, 4651-4662

Gorb E., Kastner V., Peressadko A., Arzt E., Gaume L., Rowe N. and Gorb S. Structure and properties of the glandular surface in the digestive zone of the pitcher in the carnivorous plant Nepenthes ventrata and its role in the insect trapping and retention The Journal of Experimental Biology, 2004, vol. 207, 2947-2963

Gaume L., Perret P., Gorb E., Gorb S., Labat J.-J. and Rowe N. How do plant waxes cause flies to slide? Experimental tests of wax-based trapping mechanisms in three pitfall carnivorous plants Arthropod Structure & Development, 2004, vol. 33, 103-111


Last reviewed: By John M. Grohol, Psy.D. on 21 Feb 2009
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